Original Research Article I Volume 11 I Issue 3 I 2023

Effect of Methyl Parathion (an Organophosphate) on Electrophoretic Patterns of Esterases in Gill and Muscle Tissues of Freshwater Cat fish. H. fossils (bloch)

Bheem Rao T, Venkanna Lunavath

Biolife, Volume 11, Issue 3, 2023, pp 58-63

DOI:https://doi.org/10.5281/zenodo.8380632

Abstract:

The present study was under taken to assess the toxicological effect of Methyl Parathion (an Organophosphate) on esterase isozyme banding patterns in gill and muscle tissues of freshwater cat fish Heteropneustes fossilis (Bloch) at different time intervals i.e. 24,48,72 and 96hrs and was compared with control. The esterase isozymes were quantitatively analyzed by using 7.5٪ native polyacrylamide gel electrophoresis (PAGE) stained with α-naphthyl acetate as substrate. Three different esterase bands were detected and named as Est-1; Est-2 and Est-3 with different relative mobilities such as 0.60; 0.40; 0.30 in gill tissue and 0.60; 0.40; 0.30 in Muscle. All the three esterase bands were found in gill and muscle tissues. Among the three esterases Est-1 at 24hrs and Est-2 at 48 hrs and 72hrs in gill tissue, Est-2 at 24hrs in muscle tissue is found to be more abundant with highest intensity. The intensity of Est-3 was faintly stained in both the tissues.

Keywords:

Esterase, isozymes, PAGE, H.fossilis, Methyl Parathion

References:

1. Magar R.S, Afsar Shaikh. Effect of Malathion on acid phosphatase activity of fresh water fish Channa punctatus. IJPCBS. 2013; 3(3), 720-722.
2. Pritchard JB. Aquatic toxicology: Past, present, and prospects. Environmental Health Perspectives, 1993; 100:249-257.
3. Jayaprakash G, Raju N, Bheem Rao T, Venkaiah Y. Effect of Malathion (an Organophosphate) on Biochemical constituents Of fresh Water Cat Fish Heteropneustes fossilis (Bloch). IJBPAS, February, 2015; 4(2): 668-677.
4. Sarkar B, Chatterjee A, Adhikari S, Ayyappan S. Carbofuran and cypermethrin- induced histopathological alterations in the liver of Labeo rohita (Hamilton) and its recovery, App Ichthyol, 2005; 21: 131.
5. Prakasam A, Sethupathy S, Lalitha S. Plasma and RBCs antioxidant status in occupational male pesticide sprayers. Clinic. Chim.Acta., 2001; 310:107-112.
6. Storm JE, Karl KR, Doull J. Occupational exposure limits for 30 organophosphate pesticides based on inhibition of red blood cell acetylcholinesterase. Toxicology, 2000; 150: 1-29.
7. Humphrey CA, Klumpp DW, Raethke N. Ambon Dansel (Pomancentrus amboinensis) as a bioindicator organism for the great Barrier Reef: responses to chlorpyrifos. Bulletin of environmental contamination and toxicology, 2004; 72, 888- 895.
8. Callaghan A, Boiroux V, Raymofld M, Pasteur N. Prevention of changes in electrophoretic mobility of overproduced esterase from organophosphate-resistant mosquitoesof the Culexpipiens complex. Med. Vet. Entomol. 1994; 8: 391-394.
9. Abdur Rashid Md . Esterase banding pattern in different age groups of mosquito fish, Gambusia
10. Joshi VM, Desai AK. Effect of sublethal concentration of monochrotophos on acid and alkaline phosphatase activities in the tissues of fresh water fish Tilapia mossambica J. Anim.Morphol.Physiol. 1981; 28: 22.
11. JaroliDP, Sharma BL.Effect of Organophosphate Insecticide on the Organic Constituents in Liver of Channa punctatus. Asian J. Exp. Sci,2005; 19(1): 121-129.
12. Sreenivasan RS, Krishna Moorthy P, Deecaraman M. Effect of Phosphatases Activity in the Hepatopancreas and Muscle of the Fresh Water Female Field Crab, Spiralothelphus ahydrodroma (Herbst) Treated with Cypermethrin, Int. J. of Pharma. Sci. And Drug Res. 2011; 3(2): 123-126.

Article Dates:

Received: 19 July 2023; Accepted: 30 August 2023: Published online: 26 September 2023

How To Cite:

Bheem Rao T, & Venkanna Lunavath. (2023). Effect of Methyl Parathion (an Organophosphate) on Electrophoretic Patterns of Esterases in Gill and Muscle Tissues of Freshwater Cat fish. H. fossils (bloch). Biolife, 11(3), 58–63. https://doi.org/10.5281/zenodo.8380632