Original Research Article I Volume 11 I Issue 2 I 2023

Anti-Diabetic Activity of Selected Medicinal Plant Extracts Used By Tribals in the Adilabad District of Telangana State By in Vitro

Priyanka C

Biolife, Volume 11, Issue 2, 2023, pp 72-78

DOI: https://dx.doi.org/10.5281/zenodo.7855153


Diabetes mellitus is a metabolic condition marked by high blood glucose levels as well as changes in carbohydrate, lipid, and protein metabolism. The goal of this study was to determine the inhibitory effect of selected plants (Artemisia vulgaris - root, and Angelica archangelica - leaves) on -glucosidase and -amylase to assess anti-diabetic effectiveness in vitro. By using the Soxhlet extraction method, each plant powder was repeatedly extracted with different organic solvents of increasing polarity. To investigate invitro anti-diabetic efficacy, the various solvent extracts were submitted to a -glucosidase and -amylase enzyme inhibition assay. Artemisia vulgaris stem bark crude extracts yielded 22.43 percent, 25.56 percent, 10.14 percent, 12.12 percent, and 1.10 percent in hexane, chloroform, ethyl acetate, acetone, and methanol, respectively. Angelica archangelica extracts were shown to have IC50 values of 57 g/ml, 43 g/ml, 63 g/ml, 64 g/ml, and 70 g/ml in n-hexane, chloroform, ethyl acetate, acetone, and methanol, respectively. In a dose-dependent way, all extracts reduced enzyme activity. Based on the IC50 values, Angelica archangelica chloroform extract was the most active of the two plant species, followed by Artemisia vulgaris. The chemicals responsible for A. archangelica promise in vivo anti-diabetic effect should be studied further


Angelica archangelica, Artemisia vulgaris, alpha-amylase, alpha-glucosidase


  1. Adisakwattana S, Lerdsuwankij O, Poputtachai U, Minipun A, Suparpprom C: Inhibitory activity of cinnamon bark species and their combination effect with acarbose against intestinal α -glucosidase and pancreatic α-amylase. Plant Foods Hum Nutr 2011, 66:143 – 148.
  2. Alberti KGMM, Zimmet PZ: Definition, diagnosis and classification of diabetes mellitus and its complications. Report of a WHO Consultation Geneva: WHO, 1999.
  3. Ali, H., Houghton, P.J., Soumyanath, A., 2006. -Amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. Journal of Ethnopharmacology 107, 449–455.
  4. Asano, N., Ikeda, K., Kasahara, M., Arai, Y., Kizu, H., 2004. Glycosidase-inhibiting pyrrolidines and pyrrolizidines with a long side chain in Scilla peruviana. Journal of Natural Products 67, 846–850.
  5. Conforti, F., Statti, G., Loizzo, M.R., Sacchetti, G., Poli, F., Menichini, F., 2005. In Vitro antioxidant effect and inhibition of α-amylase of two varieties of Amaranthus caudatus seeds. Biological and Pharmaceutical Bulletin 28, 1098–1102.
  6. E. Sucharitha and M. Estari. 2013. Evaluation of antidiabetic activity of medicinal plant extracts used by tribal communities in rural areas of Warangal district, Andhra Pradesh. Biology and Medicine, 5 (20-25).
  7. Gerrits PM, Tsalikian E: Diabetes and fructose metabolism. Am J Clin Nutr 1993, 58:796S – 799S.
  8. Ghosal S, Tripathi VK, Chauhan S. Active constituent ofEmblica officinalis: Part I. The chemistry and antioxidant effects of two new hydrolysable tannins, emblicanin A and B. Indian J Chem 1996;35:941-8
  9. ‐cyanogenic pyridone glucosides from Angelica archangelica (Euphorbiaceae). Phytochemistry 2009; 70 (2): 270‐277 
  10. Koh LW, Wong LL, Loo YY, Kasapis S, Huang D: Evaluation of different teas against starch digestibility by mammalian glycosidases. J Agric Food Chem 2010, 58:148 – 154.....

Article Dates:

28 February 2023; Accepted: 11 March 2023; Published: 22 April 2023

How To Cite:

Priyanka. (2023). Anti-Diabetic Activity of Selected Medicinal Plant Extracts Used By Tribals in the Adilabad District of Telangana State By in Vitro. Biolife, 11(2), 72–78. https://doi.org/10.5281/zenodo.7855153

Download Full Paper: PDF