Original Research Article I Volume 1 I Issue 2 I 2014

Analysis of Micronuclei Formation is an Indicator of Genotoxicity Assessment to Acrylamide in Rats and Developing Chick Embryos.

Venkatasubbaiah

Biolife; 2013, 1(2), pp 35-44

DOI:https://doi.org/10.5281/zenodo.7185294

Abstract:

The formation of micronuclei (MN) is a widely used and accepted endpoint of genotoxicity testing. This assay provides a simple and direct measure of the induction of structural or numerical aberrations to chromosomes. In this study we describe about acrylamide treated rats and chick eggs with different doses like 0.1, 0.2 and 0.3mgs to eggs and 16, 32, 48 mgs to rats for 24,48 and 72hrs, for the detection of micronucleus formation in reticulocytes.

Keywords:

Acrylamide, Micronuclei. Radar data analysis, doughnut data presentation, rat, embryo, reticulocytes.

References:

 1.      Abramsson-Zetterberg L., (2003) The dose-response relationship at very low doses of acrylamide is linear in the flow cytometerbased mouse micronucleus assay, Mutat. Res; 535: 215–222.

2.      Adler I.D., Reitmer P. Schmöller R.,  (1994) Dose response for heritable translocations induced by acrylamide in spermatids of mice. Mutat. Res; 309: 285–291.

3.      Adler I.D., Zouth R. and Schmid E., (1993) Perturbation of cell division by acrylamide in vitro and in vivoMutat.  Res; 301: 249–254.

4.      Angelo Carere. (2006)  Genotoxicity and Carcinogenicity of acrylamide: a critical review. Ann. 1st Super Sanita., 42 (2) 144- 155.

5.      Bloom S.E. (1978) Chick embryos for detecting environmental mutagens. In: A.Hollaender, F.J. de Serres (Eds), Chemical mutagens: Principles and Methods for their detection. Plenum, New York. 5-203-232.

6.      Cammerer A., Elhajouji A. and Suter W., (2008) In vivo micronucleus test with flow cytometry after acute and chronic exposures of rats to chemicals, Mutat. Res; 62(6):26–33.1.      irradiated Swiss mice. Int J Radiat Biol.; 63:239–245.

8.      Dearfield K., Douglas L., George R., Ehling U.H., Moore M.M., Sega G.A.,  (1995) Acrylamide: a review of its genotoxicity and an assessment of heritable genetic risk. Mutat Res; 330: 71–99.

9.      Dieterlen-Lievre F. Birds, In: A.F.Rourley and N.A.Ratcliffe , (1988) Vertebrate blood cells, Cambridge 257-336.

10.      Food and Agricultural organization, World Health Organization. (2002) Consultation on the Health implication of Acrylamide in Food. Geneva, FAO, WHO.

11.      Hart J.W and Engberg-pederson H. (1983) Statistics of the mouse bone marrow micronucleus test: counting, distribution and evaluation of results.  Mutat. Res., 111195-207.

12.      Hayashi M., MacGregor J.T., Gatehouse D.G., Blakey D.H., Dertinger S.D., Abramsson L., Zetterberg L., Krishna G., Morita T., Russo A., Asano N., Suzuki H., Ohyama W., and Gibson D., (2007) In Vivo Micronucleus Assay Working Group, IWGT. In vivo erythrocyte micronucleus assay III. Validation and regulatory acceptance of automated scoring and the use of rat peripheral blood reticulocytes with discussion of non-hematopoietic target cells and a single dose level limit test. Mutat Res.; 627: 10–30....

 

 

 

Article Dates:

Received: 12 April 2013; Accepted: 26 May 2013; Published: 8 June 2013

How To Cite:

Venkatasubbaiah. (2022). Analysis of Micronuclei Formation is an Indicator of Genotoxicity Assessment to Acrylamide in Rats and Developing Chick Embryos. Biolife, 1(2), 35–44. https://doi.org/10.5281/zenodo.7185294

Download Full Paper: PDF

Logo


Logo