Original Research Article I Volume 3 I Issue 2 I 2015

The evaluation of Isolated KH1-29 properties for producing α-amylase from a novel native highly thermostable, thermophilic and alkaline Bacillus sp

Vahid Danesh; Numan Ozcan; Mojtaba Najafi

Biolife; 2015, 3(2), pp 571-575

DOI: https://doi.org/10.5281/zenodo.7270814

Abstract:

Alkaliphilic and thermophilic bacillus sp. KH1-29 which was isolated from Caspian Lake of Iran, produced thermostable α-Amylase at 55°C, pH 8.0. The molecular weight of the enzyme was estimated 45 kilodallton using sodium dodecyl sulphate-polyacrylamide gel electrophoresis.

Keywords:

Isolation, Bacillus sp., Thermophile, Alkaline, α-Amylase, Molecular weight

References:

  1. Alghabpoor, S.S., Panosyan, H. and Popov, Y. (2013). Purification and characterization of a novel thermostable and acid stable α-amylase from Bacillus Sp. Iranian S1. Int J Eng 26(8): 815-820.
  2. Al-Quadan, F., Akel. H. and Natshi, R. (2009). Characteristics of a novel highly thermostable and extremely thermophilic alkalitolerant amylase from hyperthermophilic Bacillus strain HUTBS51. OnLine J Biol Sci 9(3): 67- 74.
  3. Amal A. Hassan, Esam H. Mansour, Abo El-Fath A. El Bedawey and Mohamed S. Zaki (2015). Effect of α-amylase enzyme on rheological properties and quality of betifore-type cookies. Biolife 2015; 3(1); 31-39.
  4. Arikan, B., Unaldi, N., Coral, G., Colak, O., Aygan, A. and Gulnaz, O. (2003). Enzymatic properties of a novel thermostable, thermophilic, alkaline and chelator resistant amylase from an alkaliphilic Bacillus sp. isolate ANT- 6. Process Biochem 38: 1397-1403.
  5. Asgher M, Asad MJ, Rahman SU, Legge RLA.(2007). thermostable α -amylase from a moderately thermophilic Bacillus subtilis strain for starch processing. J Food Eng; 79:950-955.
  6. Aygan, A., Arikan, B., Korkmaz, H., Dinçer, S. and Çolak, Ö. (2008). Highly thermostable and alkaline α-amylase from a halotolerant-alkaliphilic Bacillus sp. AB68. Braz J Microbiol 39(3): 547-553.
  7. Bakhtiary, N., Hosseinkhani, S., Mohajeri-Tehrani, M. R., & Hedayati, M (2014). Critical role of plasma C-peptide on control of ATP/ADP ratio of RBC. The Ame J Sci & Med Res, 1(1), 7-15.
  8. Fehimeh, M., Nima, B. and Majid, B. (2013). Isolation, characterization and identification of amylase producing halothermophilic isolates from Howz Soltan Lake, Iran. Afr J Microbiol Res 7(36): 4483-4490.
  9. Gümüşel F. 2002.  Biyoteknoloji, genetik ve sağlık sektörü.Kocaeli Sanayii İçin Teknolojik Uzgörü Ortak Projesi, 73-135.
  10. Hamilton LM, Kelly CT, Fogarty WM et al. (1999). Production and properties of the raw starch-digesting a-amylase of Bacillus sp. IMD 435. Process Biochem 35: 27–31.
  11. Igarashi, K., Hatada, Y., Hagihara, H., Saeki, K., Takaiwa, M., Eumura, T., Ara, K., Ozaki, K., Kawai, M., Kobayashi, T. and Ito, S. (1998). Enzymatic properties of a novel liquefying a-amylase from an alkaliphilic Bacillus isolate and entire nucleotide and amino acid sequences. Appl Environ Microbiol 64(9): 3382-3389.
  12. Joo MH, Hur SH, Han YS, Kim JY. (2007). Isolation, identification, and characterization of Bacillus strains from the traditional Korean soybean-fermented food, Chungkookjang. J Appl Biol Chem 2007; 50:202-210...

Article Dates:

Received: 8 April 2015; Accepted: 20 May 2015; Published: 9 June 2015

How To Cite:

Vahid Danesh, Numan Ozcan, & Mojtaba Najafi. (2022). The evaluation of Isolated KH1-29 properties for producing α-amylase from a novel native highly thermostable, thermophilic and alkaline Bacillus sp. Biolife, 3(2), 571–575. https://doi.org/10.5281/zenodo.7270814

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