Original Research Article I Volume 10 I Issue 4 I 2022
Biochemical profile of liver of Indian major carp (Labio rohita) exposed to immunostimulant and bacterial infection
Ankamma, N.and Viveka Vardhani, V
Biolife; 2022, 10(4), pp 8-15
DOI:https://dx.doi.org/10.5281/zenodo.7268301
Abstract:
The efficacy of oral feeding diet (supplement) Aqua Fix was studied on the biochemical changes in liver and immune response with regard to biochemical profile against aeromoniasis in L.rohita. Three groups(A,B,C) of six months old fish were employed; groups A and B were treated with Aqua Fix for 4 days, on day 5 fish of group B and C were infected with Aeromonas hydrophila @ 10-6CFU/fish(fish of group C were fed with normal diet for 4 days).Controls (group D) were untreated and uninfected. Necropsies were made on day 1, 2,3,4,7 and 15 after infection in fish of group B and C; fish of group A and D were also necropsied on same designated days. Liver protein, carbohydrate, DNA and RNA were estimated following standard methods and results were subjected to statistical analysis and found that the immunostimulant modulated immunity to enhance the level of protein, carbohydrate, DNA and RNA in liver. Stress caused by aeromoniasis led to the decrease of all the above biomolecules in fish which received infection (group C)
Keywords:
Liver, Immunostimulant , Aeromonas hydrophila , Labeo rohita.
References:
- Anbazahan, S.M., Mari, LS.S., Yogeshwari, G., Jagrutho, C., Thirumurgan, R., Arockiaraj, J., Velanganni, A.A.J., Krishnamoorthy, P., Balasundara, C. and Harikrishnan, P. 2014. Immune response and disease resistance of carotenoids diet in Cyprinus carpio against Aeromonas hydrophila. Fish & Shellfish Immunol. 40: 9-13.
- Awad, E. and Austin, B. 2010. Use of lupin, Lupinus perpenis, mango, Mangifera indica and stinging nettle, Urtica dioica, as feed additives to prevent Aeromonas hydrophila infection in rainbow trout, Oncorhynchus mykiss, Walbaurnt, J. Fish. Dis. 33: 413-420.
- Baba, T., Watase, Y. and Yoshinaga, Y. 1993. Activation of mononuclear phagocytes function by levamisole immersion in carp. Nippon Suisan Gokkaishi, 59: 301-307.
- Baba, E., Acar, U., Ontas, C., Kesbic, O.S. and Yilmaz, S. 2016. The use of Avena sativa extract against Aeromonas hydrophila and its effect on growth performance, hematological and immunological parameters in common carp (Cyprinus carpio). Ita. J. Ani. Sci. 15(2): 325-333
- Binaii, M, Ghiasi, M, Farabi, S.M.V., Ourgholam, R, Fazli, H, Safari, R, Alavi, S.E, Taghavi, M.J. and Bankehsaz, Z. 2014. Biochemical and hemato-immunological parameters in juvenile beluga (Huso huso) following the diet supplemented with nettle (Urtica dioica). Fish & Shellfish Immunol. 36: 46–51.
- Burton, K. 1956. A study of the conditions and mechanisms of the diphenylamine reaction for the colorimetric estimation of deoxyribose-nuclic acid. Biochem. J. 62: 315.
- Citarasu, T., Babu, M.M., Sekar, R.R.J. and Marian, M.P. 2002. Developing Artemia enriched herbal diet for producing quality larvae in Penaeus monodon, Fabricius. Asian Fisheries Sci. 15: 21–32.
- Gangwar, G., Rajesh Singh, Rao, A.P. and Tewari, S.C. 2007. Changes in biochemical composition of muscles of Indian major carp Labeo rohita (Ham.) in influence of age. The Asian J. Ani. Sci. 2(1 and 2): 46-48.
- Gopalakannan, A. and Venkatasan, A. 2006. Immunomodulatory effect of dietary intake of chitin, chitosan and levamisole on the immune system of Cyprinus carpio and control of Aeromonas hydrophila infection in ponds. Aquaculture, 255: 179-187.
- Harikrishnan, R. and Balasundaram, C. 2005. Modern trends in Aeromonas hydrophila disease management with fish. Rev. Fish Sci. 13: 281-320.....
Article Dates:
Received: 19 August 2022; Accepted: 12 October 2022; Published: 31 October 2022
How To Cite:
Ankamma, N, & Viveka Vardhani, V. (2022). Biochemical profile of liver of Indian major carp (Labio rohita) exposed to immunostimulant and bacterial infection. Biolife, 10(4), 8–15.https://dx.doi.org/10.5281/zenodo.7268301
