Original Research Article I Volume 10 I Issue 4 I 2022

Biochemical Indices in Gills and Intestine of Labeo rohita (Indian major carp) treated with Immunomodulator and Aeromonas hydrophila

Subba Reddy, K.V; Simhachalam, G

Biolife.2022, 10(4), pp 1-7

DOI:https://doi.org/10.5281/zenodo.7245439

Abstract:

The present study was conducted to investigate the biochemical changes in the gills and intestine of Labeo rohita treated with Aqua Fix and Aeromonas hydrophila. 3 groups (A, B, C) of experimental and 1 group (D) of control fish (6 months old) were selected for investigations. Two groups of fish (A, B) were treated with Aqua Fix for 4 days, fish of group B and C were infected with A. hydrophila @ 10-6 CFU/fish on day 5 (normal feed was given to fish of group C). Controls (group D) were untreated and uninfected. Tissue samples were collected on day 1, 2, 3, 4, 7 and 15 after infection from fish of group B and C, and also from groups A and D on the same designated days. Protein and DNA from gills and intestine were estimated following standard methods. Statistical analysis on gill protein and DNA, and muscle protein and DNA showed significant levels in immunomodulated fish (Group A) compared to that of immunomodulated + infected (Group B), infected (Group C) and control fish (Group D) indicating that the treated immunostimulant (Aqua Fix) might have modulated the immunity resulting in the increase of biomolecules It is also clear that aeromoniasis might influence the lysis of protein in gill and intestine leading to the low level of protein and DNA in gills and intestine of fish in groups B and C

Keywords:

Aeromonas hydrophila, Labeo rohita, gills, Intestine, Protein, DNA.

References:

  1. Aathi, K., Ramasubramanian, V., Uthayakumar, V. and Munirasu, S. (2013). Effect of chitosan supplemented diet on survival, growth, haematological, biochemical and immunological responses of Indian major carp Labeo rohita.  Int. Res. J. Pharm. 4(5): 141-147.
  2. Anderson, D.P., Siwicki, A.K. 1995. Basic haematology and serology of fish health programmes. In: Diseases in Asian aquaculture II. M. Shariff, J.R. Srthur and R.P. Subasinghe (Eds.). Fish. Health. Section, Asian Fisheries Society, Manila, Philippines, pp.185-202.
  3. Anil Kumar, V., Janaiah, C. and Venkateswarlu,P. (2010). Effect of thiamethoxan alters serum biochemical parameters in Channa punctatus (Bloch). Asian. J. Bio. Sci. 5: 106-110.
  4. Baba, E., Acar, U., Ontas, C., Kesbic, O.S. and Yilmaz, S. (2016).  The use of Avena sativa extract against Aeromonas hydrophila and its effect on growth performance, hematological and immunological parameters in common carp (Cyprinus carpio). Ita. J. Ani. Sci. 15(2): 325-333.
  5. Ellsaesser, C.F. and Clam, L.W. (1986).  Haematological and immunological changes in channel catfish stressed by handling and transport. J. Fish Biol. 28: 511-521.
  6. Fallahpour, F., Banaee, M., Javadzade, N. (2014).  Effects of dietary marshmallow (Althaea officinalis L.) extract on growth performance and body composition of common carp (Cyprinus carpio). Int. J. Adv. Biol. Biom. Res. 2(8): 2453-2460.
  7. Gupta, S.K., Pal, A.K. Sahu, N.P., Dalvi, R., Kumar, V. and Mukherjee, S.C. (2008).  Microbial levan in the diet of Labeo rohita Hamilton juveniles effect on non-specific immunity and histopathological changes after challenge with Aeromonas hydrophila. J. Fish Dis. 31: 649-657.
  8. Islam, M.T., Rashid, M.M. and Mostafa, K. (2008). Histopathological studies of experimentally infected shing, Heteropneustes fossilis with Aeromonas hydrophila bacteria. Progress. Agric. 19(1): 89-96.
  9. Jeyakumari, Sahoo, P.K and Giri (2007). Effects of polyherbal formulation ‘Immu- Plus’ on immunity and disease resistance of Indian major carp, Labeo rohita at different stages of growth. Indian J. Exp. Biol. 3: 291-298.
  10. Lowry, O.H., Rose Brough, N.J., Fare, A.L. and Randall, R.J. (1951).  Protein measurement with folin phenol reagent. J. Biol. Che. 193: 265-275.
  11. Lunden, T. and Bylund, G. (2000). The influence of in vitro and in vivo exposure to antibiotics on mitogen induced proliferation of lymphoid cells in rainbow trout  (Oncorhynchus mykiss). Fish Shellfish Immunol. 10: 395-404..........

Article Dates:

Received: 11 August 2022; Accepted: 2October 2022; Published online: 24 October 2022

How To Cite:

Subba Reddy, K.V, & Simhachalam, G. (2022). Biochemical Indices in Gills and Intestine of Labeo rohita (Indian major carp) treated with Immunomodulator and Aeromonas hydrophila. Biolife, 10(4), 1–7. https://doi.org/10.5281/zenodo.7245439

Download Full Paper: PDF
Submit Manuscript
Join as Reviewer / Editor

Logo


Logo