Original Research Article I Volume 3 I Issue 4 I 2015

Effect of Various Growth Hormone Concentration and Combination on Protocol Optimization for In vitro Rapid Propagation of Withania somnifera

Indrani Trivedi; Maheshwar Prasad Trivedi; Upendra Kishore Sinha; Anupama; Sanjeev Kumar Ambasta

Biolife; 2015, 3(4), pp 889-896

DOI:https://doi.org/10.5281/zenodo.7306856

Abstract:

Withania somnifera(l.) Dunal is commonly known as ashwagandha and belongs to family Solanaceae. The plant is an erect undershrub with long tuberous roots. All the parts of the plants have shown remarkable significance in the field of pharmacology. The major chemical constituents of this plant is withanolide A and D. These are anti-inflammatory and enhance the bodies defence against infections and tumours. Generally leaves and roots are used as drug. Multiple uses of the plant have made it an endangered species.

Keywords:

Shrivastava, S., Dubey, P.K. 2007. In vitro callus induction and shoot regeneration in Withania somnifera Dunal. International Journal of Biotechnology and Biochemistry. 24: 10-19. Tyler V.e., Brady L.R. and Robbers J.E. (1981). Pharmacognosy. Lea and Febiger, Philadelphia P-520. Singh Sandhya and Kumar Sushil (1998). Withania somnifera, The Indian Ginseng Ashwagandha, Central Institute of Medicinal and Aromatic Plants (CIMAP) Lucknow- 226015, India. Jaffer H.J., Javed A.l.M., Saber H.s. and Al-Naib A. (1988): Evaluation of antimicrobial activity of Withania somnifera extracts, Fitoterapia, 59: 497-500. Devi P.U. and Sharada A.C. (1992). In vitro growth inhibitory effect of Withania somnifera (Ashwagandha) on a transplantable mouse tumour sarcoma 180, Indian J Exp Biol 30: 169-172. Kirtikar, K.R. and Basu, B.D. (1975). Indian Medicinal Plants, Vol. 3 (Bishen singh, Mahendra Pal Singh, Dehradun): 1774-1777. Shukla,Durga Dutt,Bhattarai,Nabin and Pant,Bijiya (2010).Nepal journal of Science and Technology,11:.101-106 Sivanesan, I. (2007). Direct regeneration from apical bud explants of Withania somnifera Dunal. Indian Journal of Biotechnology, 6: 125-127. Rao, Y.V. Ravi Shankar A., Lakshmi T.V.R. and raja Rao, K.G., (2004). Plant Regeneration in Physalis pubescens and its induced mutant. Plant Tissue Culture. 15 (1): 9-15. Murashige, T. and F. Skoog. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497. Rani, Gita and grover I.S. 1999. In vitro callus induction and regeneration studies in Withania somnifera. Plant cell. Tissue and Organ culture 77: 23-27. Koch, K.E. 1969. Carbohydrate-modulated gene expression in plant, A Rev. Pl. Physiol. Pl. Mol. Biol. 47: 509-540.

References:

  1. Shrivastava, S., Dubey, P.K. 2007. In vitro callus induction and shoot regeneration in Withania somnifera Dunal. International Journal of Biotechnology and Biochemistry. 24: 10-19. 
  2. Tyler V.e., Brady L.R. and Robbers J.E. (1981). Pharmacognosy. Lea and Febiger, Philadelphia P-520.
  3. Singh Sandhya and Kumar Sushil (1998). Withania somnifera, The Indian Ginseng Ashwagandha, Central Institute of Medicinal and Aromatic Plants (CIMAP) Lucknow- 226015, India.
  4. Jaffer H.J., Javed A.l.M., Saber H.s. and Al-Naib A. (1988): Evaluation of antimicrobial activity of Withania somnifera extracts, Fitoterapia, 59: 497-500.
  5. Devi P.U. and Sharada A.C. (1992). In vitro growth inhibitory effect of Withania somnifera  (Ashwagandha) on a transplantable mouse tumour sarcoma 180, Indian J Exp Biol 30: 169-172.
  6. Kirtikar, K.R. and Basu, B.D. (1975). Indian Medicinal Plants, Vol. 3 (Bishen singh, Mahendra Pal Singh, Dehradun): 1774-1777.
  7. Shukla,Durga Dutt,Bhattarai,Nabin and Pant,Bijiya (2010).Nepal journal of Science and Technology,11:.101-106
  8. Sivanesan, I. (2007). Direct regeneration from apical bud explants of Withania somnifera Dunal. Indian Journal of Biotechnology, 6: 125-127.
  9. Rao, Y.V. Ravi Shankar A., Lakshmi T.V.R. and raja Rao, K.G., (2004). Plant Regeneration in Physalis pubescens and its induced mutant. Plant Tissue Culture. 15 (1): 9-15.
  10. Murashige, T. and F. Skoog. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
  11. Rani, Gita and grover I.S. 1999. In vitro callus induction and regeneration studies in Withania somnifera. Plant cell. Tissue and Organ culture 77: 23-27.
  12. Koch, K.E. 1969. Carbohydrate-modulated gene expression in plant, A Rev. Pl. Physiol. Pl. Mol. Biol. 47: 509-540....

Article Dates:

Received: 4 October 2015; Accepted: 25 November 2015; Published: 8 December 2015

How To Cite:

Indrani Trivedi, Maheshwar Prasad Trivedi, Upendra Kishore Sinha, Anupama, & Sanjeev Kumar Ambasta. (2022). Effect of Various Growth Hormone Concentration and Combination on Protocol Optimization for In vitro Rapid Propagation of Withania somnifera. Biolife, 3(4), 889–896. https://doi.org/10.5281/zenodo.7306856

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