Original Research Article I Volume 11 I Issue 1 I 2023

Electrophoretic Banding Pattern of Esterase Isozymes In Fresh Water Fish Labeo rohita

Venkteswara Rao Mandalapu, Venakiah Yanamala

Biolife, Volume 11, Issue 1, 2023, pp 57-61

DOI:https://doi.org/10.5281/zenodo.7747466

Abstract:

Labeo rohita is most preferred indigenous edible species in most of the countries and the most abundant species in our fresh water rivers, paddy fields and other water channels. Fishes are the excellent models for monitoring environmental contamination in aquatic system. The In our present investigation electrophoretic banding patterns of tissue specific esterases in various tissues i.e. gill, liver, intestine, muscle and brain were investigated in fresh water fish Labeo rohita. The results revealed that the electrophoretic esterase banding patterns varied in different tissues i.e. gill, liver, intestine, muscle and brain of fish Labeo rohita. Esterase Isozyme patterns were separated on thin layer 1.5 mm (thickness) polyacrylamide gels (SDS-7.5%) and stained with α – naphthyl acetate used as substrate. Three different esterase bands were detected and named as Est-1, Est-2 and Est-3 with different relative motilities (Rm) such as 0.6± 0.05; 0.4± 0.05; 0.3± 0.05. All the three esterase bands were present in all tissues i.e. gill, liver, intestine, muscle and brain. Among the all tissues gill and liver tissue exhibited deeply staining of Est-1; Est-2 and Est-3(+++).

Keywords:

Labeo rohita, Esterase Isozymes, Polyacrylamide gels, α-naphthylacetate, Electrophoretic banding patterns. Relative mobilites. (Rm)

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Article Dates:

Received: 2 5 January 2022; Accepted: 2 8 February 2023; Published online: 1 8 March 2023.

How To Cite:

Venkteswara Rao Mandalapu, & Venakiah Yanamala. (2023). Electrophoretic Banding Pattern of Esterase Isozymes In Fresh Water Fish Labeo rohita. Biolife, 11(1), 57–61. https://doi.org/10.5281/zenodo.7747466

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