Original Research Article I Volume 4 I Issue 2 I 2016

The effect of Aeromoniasis on protein and DNA content and histopathology of muscle and gill in adaptively immunized fish

Satyalatha B.D.J; Viveka Vardhani. V

Biolife; 2016, 4(2), pp 364-369.

DOI: https://doi.org/10.5281/zenodo.7318327


The present investigations were designed to estimate the content of protein and DNA and to understand the histopathology in muscle and gill of fish infected with repeated doses of A. liquefaciens. Two varied multiple doses of pathogen, 10-2 + 10-2 CFU/Fish and 10-3 + 10-3 CFU / Fish were injected intramuscularly to two different groups of fish A and B (66 in each group) respectively; two groups a and b were served as controls for comparison. Muscle and gill were selected for studying the content of protein and DNA and for histopatholgoical changes


Aeromoniasis, protien, DNA, gill, muscle, fish.


  1. Ali, K.S., Dorgai, L., Gazdag, A., Abraham, M. and Hermesz, E. (2003).  Identification and induction of hsp 70 ene by heat shock and cadmium exposure in carp. Acta. Biol. Hung, 54(3-4): 323-334.
  2. Anderson, D.P.; Siwicki, A.K., 1995; Basic haematology and serology for fish health programs. In: Diseases in Asian aquaculture II. M. Shariff, J.R. Arthur and R. P. Subasinghe (Eds). Fish Health Section. Asian Fisheries Scoeity, Manila, Philippines, pp. 185-202. I
  3. Begum, G. (2004).  Carbofuran insecticide induced biochemical alterations in liver and muscle tissues of the fish Clarias batrachus (Linn.) and recovery response. Aquatic Toxicol., 66(1): 83-92.
  4. Boone, A.N., and Vijayan, M.M. (2002).  Constitutive heat shock protein 70 (HSC 70) expression in rainbow trout hepataytes: effect of heat shock and heavy metal exposure. Comp. Biochem. Physiol. Toxicol. Pharmacol., 132(2): 223-233.
  5. Dash, K.; Sahu, A.; Gangal, S.V., 1993: Natural serum haemagglutinins (lectins) in fish : physicochemical characterization. Fish Shelifish Immunol. 3, 345-360. I
  6. Elnemaki, E. and Abuzinadah, O. (2003).  Effect of contra/insect 500/50 E.C. on the histopathology of Oreochromis spilurus fish.  Egypt. J. Aquat. Res. Fish. 29: 221-253.
  7. Huizinga, H.W., Esch, G.W. and Hazen, T.C. (1979).  Histopathology of red-sore disease (Aeromonas hydrophila) in naturally and experimentally infected largemouth bass Micropterus salmoides. J. Fish Dis. 2: 263-277.
  8. Ingram, G.A., 1980: Substances involved in the natural resistance of fish to infection – a review. J. Fish Biol. 16, 23-60. I
  9. Karunasagar, I., Rosalind, G. and Karunasagar, I. (1991).  Immunological responses of the Indian major carps to Aeromonas hydrophila vaccine. J. Fish. Dis. 14: 413-417.
  10. Lamers, C.H.J. and W.B., Van Muiswinkel, 1986.  Natural and acquired agglutinin to Aeromonas hydrophila in carp. Cyprinus carpio. Can. J. Fish Aquat. Sci. 43: 619-624.
  11. Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951).  Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265-275.
  12. Marsden, M.J.; Freeman, I.C. Cox, D; Secombes, C.J., 1996; Non-specific immune responses in families of Atlantic salmon, Salmonsalar, exhibiting differential resistance to furunculosis. Aquaculture 146, 1-16...


Article Dates:

Received: 6 April 2016; Accepted: 28 May 2016; Published: 7 June 2016

How To Cite:

Satyalatha B.D.J, & Viveka Vardhani. V. (2022). The effect of aeromoniasis on protein and DNA content and histopathology of muscle and gill in adaptively immunized fish. Biolife, 4(2), 364–369. https://doi.org/10.5281/zenodo.7318327

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