Original Research Article I Volume 4 I Issue 3 I 2016

Invivo and Invitro studies on carboxy terminal domains of leptospiral immunoglobulins of L. interrogans Icterohaemorrhagiae

S. Senthil Kumar; M.Parthiban; P. Agastian

Biolife; 2016, 4(3), pp 521-529

DOI:https://doi.org/10.5281/zenodo.7332898

Abstract:

Carboxy terminal domains of leptospiral immunoglobulins of rLigA and rLigB were assessed its hemolytic, cytotoxicity effects invitro and its protective efficacy was studied in hamster models. Experimental recombinant proteins of Leptospira interrogans Icterohaemorrhagiae were individually cloned, expressed in prokaryotic system. Purified expressed proteins confirmed by western blot, assessed for its protective efficacy using hamster models.

Keywords:

Leptospira recombinant protein, Leptospiral immunoglobulins, immune response, challenge studies, cytotoxic and hemolytic activity.

References:

  1. Alves, V.A., Gayotto, L.C., Brito, T.De., Santos, R.T., Wakamatsu, A., Vianna, M.R., and Sakata. E.E (1992). Leptospiral antigens in the liver of experimentally infected guinea pig and their relation to the morphogenesis of liver damage. Exp. Toxicol.  Pathol. (44): 425-434.
  2. Andre-Fontaine, G., Branger, C., Gray, A.W and Klaasen, H.L. (2003).  Comparison of the efficiacy of three commercial bacterins in preventing canine leptospirosis.  Vet. Rec. (153): 165-169.
  3. Bartlett, J.M.S. and Stirling, D. (2004).  DNA Extraction form Fungi, Yeast and Bacteria.  PCR Protocols Second Edition, Methods in Molecular Biology, Humana Press, (226): 53-54.
  4. Bernheimer, A.W. and Bey, R.F. (1986). Copurification of Leptospira interrogans serovar pomona hemolysin and sphingomyelinase C. Infect. Immun. (54): 262-264.
  5. Bulach, D.M., Kalambaheti, T., Dela pena, M.A. and Adler, B. (2000). Functional analysis of genus is the rfb locus of Leptospira borgpetersenii serovar hardjo subtype hardjobovis. Infect. Immun.  (68): 3793-3798.
  6. Choy, A.H., Kelley, M.M., Chen, T.L., Moller, A.K., Matsunga, J. and Haake., D.A. (2007).  Physiological osmotic induction of Leptospira interrogans Adhesion: LigA and LigB Bind Extra cellular Matrix Proteins and Fibrinogen. Infect. Immun. (75):  2441-2450.
  7. Croda, J., Ramos, J.G., Matsunga, J., Queiroz, A., Homma, A., Riley, L.W., Haake, D.A., Reis, M.G. and Ko, A.I. (2007).  Leptospira Immunoglobulin-like proteins as a serodiagnositc marker for acute leptospirosis.  J. Clin. Microbiol. (45): 1528-1534.
  8. Croda, J., Figueira C.P., Wunder Jr.,, A.E., Santos, C.S., Reis, M.G. and Ko, A.I. (2008).  Targeted Mutagenesis in Pathogenic Leptospira species: Disruption of the LigB gene does not affect virulence in animal models of Leptospirosis. Infect. Immun.  (76(12)) : 5826-5833.
  9. Del Real, G., Segers, R.P.A.M., Van der Zejst, B.A.M. and Gaastra, W. (1989). Cloning of a hemolysin gene from Leptospira interrogans serovar hardjo. Infect. Immun. (57): 2588-2590.
  10. Goldstein, S.F. and Charon, N.W. (1990). Multiple exposure photographic analysis of motile spirochetes. Pro. Nat. Acad.  Sci. (87): 4895-4899.
  11. Haake, D.A., Mazel, M.K., McCoy, A.M., Milward, F., Chao, G., Matsunga, J. and Wager, E.A. (1999). Leptospiral outer membrane proteins OmpL1 and LipL41 exhibit synergistic immune protection. Infect. Immun. (67): 6572-6582.
  12. Hartwig, D.D., Oliveira, T.L., Seixas, F.K., Forster, K.M., Rizzi, C., Hartleben, C.P., McBride A.J.A. and Dellagostin, O.A. (2010). High yield expression of leptospirosis vaccine candidates LigA and LipL32 in the methylotrophic yeast Pichia pastoris.  Microb. cell Fact. (9): 98...

Article Dates:

Received: 6 July 2016; Accepted: 23 August 2016; Published: 5 September 2016

How To Cite:

S. Senthil Kumar, M.Parthiban, & P. Agastian. (2022). Invivo and Invitro studies on carboxy terminal domains of leptospiral immunoglobulins of L. interrogans Icterohaemorrhagiae. Biolife, 4(3), 521–529. https://doi.org/10.5281/zenodo.7332898

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