Original Research Article I Volume 4 I Issue I 2016

Bovine prenatal sex determination using cell free fetal nucleic acid present in maternal plasma

M. Malarmathi; V.S. Vadivoo; T. Muthuramalingam; T. M. A. Senthil Kumar; A. Palanisammi

Biolife; 2016, 4(3), pp 431-436

DOI:https://doi.org/10.5281/zenodo.7319053

Abstract:

Circulating Nucleic acids (CNAs) are extracellular or cell free nucleic acids which circulate in the bloodstream of healthy pregnant animals. The presence of CNAs in maternal plasma offers the non-invasive prenatal diagnoses of pregnancy associated disorders and also gives opportunities to detect prenatal sex using circulating fetal Y chromosome specific nucleic acid sequence.

Keywords:

Cell free nucleic acid, prenatal sex determination, SRY, AMEL.

References:

  1. Bahar, B. Monahan, F.J.  Moloney, A.P. Schmidt, O. MacHugh, D.E.  and Sweeney, T. 2007. Long-term stability of RNA in post-mortem bovine skeletal muscle, liver and subcutaneous adipose tissues. BMC Molecular Biology. 8:108,   DOI: 10.1186/1471-2199-8-108.
  2. Cruz da, A.S. Silva.D.C. Costa, E.O.A. De M-Jr, P. da Silva, C.C. Silva, D.M. and da Cruza, A.D. (2012) Cattle fetal sex determination by polymerase chain reaction using DNA isolated from maternal plasma.  J. Animal Reproduction Science. 131: 49– 53.
  3. Davoudi, A. Tarang, A. Aleyasin, S.A. Salehi, A. Seighalani, R. and Tahmoressi F. 2012. Evaluation of two DNA extraction methods from maternal plasma for using in non-invasive bovine fetus gender determination. Iran J Reprod Med 10(6): 523-530.
  4. Fleischhacker, M. and Schmidt, B. 2007. Circulating nucleic acids (CNAs) and cancer—a survey. Biochim Biophys Acta. 1775:181–232.
  5. Gokulakrishnan, P.  Kumar, R. R. Sharma, B. D. Mendiratta, S. K. Sharma, D. and Malav, O. P. 2012. Sex determination of cattle meat by polymerase chain reaction amplification of the Amelogenin (AMEL X/AMEL Y) gene. J. Veterinary. World, .5(9): 526-529.
  6. Hamilton, C.K.  Favetta, L.A. Di Meo, G.P. Floriot, S. Perucatti, A. Peippo, J. Kantanen, J. Eggen, A. Iannuzzi, L. and King, W. A. 2009. Copy number variation of testis-specific protein, Y-encoded (TSPY) in 14 different breeds of cattle (Bos taurus) Sexual Development. 34:205–213.
  7. Kadivar, A. Hassanpour, H. Mirshokraei, P. Azari, M. Gholamhosseini, K. and Karami, A. 2013. Detection and quantification of cell-free fetal DNA in ovine maternal plasma; use it to predict fetal sex. J. Theriogenology. 79:  995–1000.
  8. Lee, S.H. Park, C.H. Park, J.T. Park, S.G. Lee, J.A. Suh, G.H. Oh, K.S. and Son, C.H. 2013. Confirmation of Male Specific Fetal Free RNA in Maternal Plasma and Comparison of Accuracy on the Sex Determination using Real-time PCR Method in Korean Native Cattle. J. Emb. Trans.  28(4): 343-348.
  9. Lemos, D.C. Takeuchi, P.L. Rios. Á.F.L. Araújo,  A. Lemos, H.C. and Ramos, E.S. 2011. Bovine fetal DNA in the maternal circulation: Applications and implications. J. Placenta 32:  912-913.
  10. Leon, P.M.M. Campos, V. F. Dellagostin, O.A. Deschamps, J.C. Seixas, F.K. and Collares, T. 2012. Equine fetal sex determination using circulating cell-free fetal DNA (ccffDNA). J. Theriogenology 77: 694–698.
  11. Lo, Y.M.D. Tein, M.S. Lau, T.K. Haines, C.J. Leung, T.N. Poon, P.M. Wainscoat, J.S. Johnson, P. J. Chang, A.M. and Hjelm, N. M. 1998. Quantitative analysis of fetal DNA in maternal plasma and serum: implications for noninvasive prenatal diagnosis. Am J Hum Genet. 62:768-775.
  12. Mayer, J. Beck, J. Soller, J.T. Wemheuer, W. Schütz, E. and Brenig, B. 2012. Analysis of circulating DNA distribution in pregnant and non-pregnant dairy cows J. Biology of reproduction. 88(2):29, 1–10...

Article Dates:

Received: 3 July 2016; Accepted: 18 August 2016; Published: 1 September 2016

How To Cite:

M. Malarmathi, V.S. Vadivoo, T. Muthuramalingam, T. M. A. Senthil Kumar, & A. Palanisammi. (2022). Bovine prenatal sex determination using cell free fetal nucleic acid present in maternal plasma. Biolife, 4(2), 431–436. https://doi.org/10.5281/zenodo.7319053

Download Full Paper: PDF

Logo


Logo