Original Research Article I Volume 4 I Issue 4 I 2016

Immunoprotection studies using Leptospiral recombinant proteins from L. interrogans Icterohaemorrhagiae in hamster models : A synergistic approach

S. Senthil Kumar; M.Parthiban; P. Agastian

Biolife; 2016, 4(4), pp 678-686

DOI:https://doi.org/10.5281/zenodo.7350368

Abstract:

Major immunodominant proteins of Leptospira interrogans Icterohaemorrahagiae were cloned, expressed in prokaryotic system was purified and confirmed by western blot. The protective efficacy of purified recombinant proteins were assayed synergistically with adjuvant in hamster models. Three combination were assessed for homologous protection (i.e, rLipL41, rOmpL1, rHapI and rSphH; rLipL41, rOmpL1, rLigA and rLigB; rLigA, rLigB, rHapI and rSphH) each protein at 25 µg and 37.5 µg concentration. Among the combinations tested combination of immunogloblulins (rLigA and rLigB) with hemolysins (rHapI and rSphH) confered 100% protection in both the concentration tested, that is evident through the elevated elicited antibody levels.

Keywords:

Leptospiral recombinant protein, recombinant proteins, outer membrane proteins, hemolysins, immunoglobulin like proteins, immune response, challenge studies, , homology modeling

References:

  1. Alves, V.A., Gayotto, L.C., Brito, T.De., Santos, R.T., Wakamatsu, A., Vianna, M.R. and Sakata, E.E. (1992).  Leptospiral antigens in the liver of experimentally infected guinea pig and their relation to the morphogenesis of liver damage. Exp. Toxicol. Pathol. 44(7): 425-434.
  2. Artiushin, S., Timoney, J.F., Nally, J. and Verma. A. (2004).  Host inducibile immunogenic Sphingomyelinases like protein, Lk73.5, of Leptospira interrogans.  Infect. Immun. (72): 742-749.
  3. Bartlett, J.M.S. and Stirling, D. (2004). DNA Extraction form Fungi, Yeast and Bacteria. PCR Protocols Second Edition, Methods in Molecular Biology, Humana Press, (226): 53-54.
  4. Bernheimer , A.W., and Bey, R.F. 1986. Copurification of Leptospira interrogans serovar pomona hemolysin and Sphingomyelinase C. Infect. Immun. 54(1): 262-264.
  5. Bolin CA, Cassells, J.A., Zuerner, R.L. and Trueba, G. (1991). Effect of vaccination with a monovalent Leptospira interrogans  serovar hardjo type hardjo-bovis vaccine on type hardjo-bovis infection of cattle. Am. J. Vet. Res. (52): 1639-1643.
  6. Branger, C., Sonrier, C., Chatrenet, B., Klonjkowski, B. and Ruvoen-Clouet, N. (2001). Identification of the hemolysis-associated protein I as a cross-protective immunogen of Leptospira interrogans by adenovirus-mediated vaccination.  Infect. Immun. (69): 6831-6838.
  7. Branger, C., Chatrenet, B., Gauvrit, A., Aviat, F. and Aubert, A. (2005). Protection against Leptospira interrogans sensu lato challenge by DNA immunization with the gene encoding hemolysin-associated protein I.  Infect. Immun. (73): 4062-4069.
  8. Carvalho, E., Barbosa, A.S,  Gomez, R.M., Oliveira, M.L.S., Romero, E.C.,  Goncales, A.P., Morais, Z.M., Vasconcellos, S.A. and Ho, P.L. (2010).  Evaluation of the Expression and Protective Potential of Leptospiral Sphingomyelinases. Curr. Microbiol. (60): 134-142.
  9. Choy, H.A., Kelley, M.M., Chen, T.L., Moller,A.K., Matsunaga, J. and Haake, D.A. (2007).  Physiological osmotic induction of Leptospira interrogans adhesion: LigA and LigB bind extracellular matrix proteins and fibrinogen. Infect. Immun. (75): 2441-2450.
  10. Coutinho, M.L., Choy, H.A., Kelley, M.M., Matsunaga, J., Babbitt, J.T., Lewis, M.S., Aleixo, J.G. and Haake, D.A. (2011).  A LigA three domain region protects hamsters from lethal infection by Leptospira interrogans.  PLoS Negl. Trop. Dis. 5(12): e1422.
  11. Porika Raju and Estari Mamidala (2015). Anti-diabetic activity of compound isolated from
    Physalis angulata fruit extracts in alloxan induced diabetic rats. The Ame J Sci & Med Res,
    2015,1(1); Pages 1 -6. doi:10.17812/ajsmr2015.11.1.
  12. Croda, J., Ramos, J.G.R., Matsunaga, J., Queiroz, A., Homma, A., Riley, L.W., Haake, D.A., Reis, M.G. and Ko, A.I. (2007).  Leptospira immunoglobulin like proteins as a serodiagnostic marker for acute Leptospirosis. J. Clin. Microbiol. 45(5): 1528-1534...

Article Dates:

Received: 3 October 2016; Accepted: 26 November 2016; Published: 4 December 2016

How To Cite:

S. Senthil Kumar, M.Parthiban, & P. Agastian. (2022). Immunoprotection studies using Leptospiral recombinant proteins from L. interrogans Icterohaemorrhagiae in hamster models : A synergistic approach. Biolife, 4(4), 678–686. https://doi.org/10.5281/zenodo.7350368

Download Full Paper: PDF
Submit Manuscript
Join as Reviewer / Editor

Logo


Logo